Fig. 3. Effect of CR AgNPs^- on Rb⁺ influx, [K]_i, and Hb_c ODs in RBCs from adult human blood. A, B and C, Rb⁺ influx, [K]_i, and Hb_c ODs, respectively, in RBCs from adult human donors donated by the Dayton Community Blood Bank (DCBC) were subjected to the procedures described in Materials and Methods and shown in Fig. 1. During the flux period, RBCs were incubated at 37 °C for 60 min under the following conditions: CONTC (RBCs + flux media), CONT (RBCs + flux media + H₂O), NPS (RBC+ flux media+ AgNPs), and NPSC (AgNPs + flux media). Rb⁺ influx, [K]_i and Hb_c ODs were determined as described in Materials and Methods. Values are mean ± S.D for a representative experiment, n = 6 individual determinations for all the conditions tested. The statistical significance of the differences between NPS vs CONT, and CONT vs CONTC for Rb⁺ influx and [K]_i concentration (*** p<0.0001, n = 6) was determined by two sample t- test. For ODs, the statistical significance of the difference between NPS vs CONT (*** p<0.0001, n = 6) and CONT vs CONTC (** p<0.001, n = 6) was also determined by two sample t- test. Further details are discussed in the text.